ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (10): 1631-1639.doi: 10.11843/j.issn.0366-6964.2014.10.008

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Detection of Differentially Expressed Genes in Rex Rabbit Skin with Different Wool Density

LI Bing,LI Fu-chang*,ZHU Yan-li,WANG Xue-peng,WANG Chun-yang   

  1. (College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China)
  • Received:2014-03-03 Online:2014-10-23 Published:2014-10-23

Abstract:

In order to screen different expressed genes for 4-day-old rex rabbit with different wool density.The Agilent’s rabbit gene expression microarray was used to determine the differentially expressed genes.The expression patterns of selected differentially expressed genes were further indentified by quantitative real-time PCR.The results showed that a total of 188 differentially expressed genes associated with rex rabbit wool density were screened out.These genes included 72 up-regulated genes which contained 24 known function genes,and 116 down-regulated genes which contained 38 known function genes.GO analysis results revealed that the expression levels of most differentially expressed genes were related to cell proliferation,apoptosis,oxidation-reduction,lipoprotein metabolic ect.KEGG pathway analysis revealed that these differential genes were mainly involved in the signaling pathways of Wnt and PPAR,and vitamin synthesis and metabolism.In addition,the results of qRT-PCR were consistent with the results of microarray hybridization,indicating that the data collected from microarray hybridization were reliable.The results suggest that the genes,such as insulin-like growth factor I (IGF-I),vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP2),may be related to the formation of wool in rex rabbit skin.And the pathway Wnt,PPAR,synthetize and metabolism of vitamin might be participate in the process of wool development in rex rabbit skin.

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